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arithmetic mean intensity (ami) of rac1 and nox2  (Carl Zeiss)


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    Carl Zeiss arithmetic mean intensity (ami) of rac1 and nox2
    Inhibition of SPT and <t>Rac1–Nox2–ROS</t> signaling in HRECs. ( a ) SPT mRNA was quantified by qRT-PCR using β-actin as a house keeping gene. ( b ) Nox2 by using lucigenin as an electron acceptor and NADPH as a substrate and ( c ) Rac1 activity was determined by G-LISA colorimetric assay kit. ( d ) ROS levels were quantified by a fluorescence method using DCHFDA. ( e ) Transfection efficiency of SPT -siRNA was determined by quantifying the relative mRNA of SPT . Each measurement was made in duplicate, and the values are represented as mean ± SD from 3–4 cell preparations. NG and HG = 5 mM or 20 mM d -glucose; HG + PA = 20 mM d -glucose + palmitic acid; HG/ SPT -si and HG + PA/ SPT -si = SPT -siRNA transfected cells in 20 mM d -glucose or 20 mM d -glucose + palmitic acid; HG + PA/Myr = 20 mM d -glucose + palmitic acid + Myriocin; HG/SC = SPT -scrambled control RNA transfected cells in 20 mM d -glucose; L-Gl = 20 mM l -glucose. *p < 0.05 vs NG, # p < 0.05 vs HG and # **p < 0.05 vs HG + PA.
    Arithmetic Mean Intensity (Ami) Of Rac1 And Nox2, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/arithmetic+mean+intensity+(ami)+of+rac1+and+nox2/arithmetic+intensity+mean/pmc09537524-191-8-18
    Average 90 stars, based on 1 article reviews
    arithmetic mean intensity (ami) of rac1 and nox2 - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Regulation of serine palmitoyl-transferase and Rac1–Nox2 signaling in diabetic retinopathy"

    Article Title: Regulation of serine palmitoyl-transferase and Rac1–Nox2 signaling in diabetic retinopathy

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-20243-2

    Inhibition of SPT and Rac1–Nox2–ROS signaling in HRECs. ( a ) SPT mRNA was quantified by qRT-PCR using β-actin as a house keeping gene. ( b ) Nox2 by using lucigenin as an electron acceptor and NADPH as a substrate and ( c ) Rac1 activity was determined by G-LISA colorimetric assay kit. ( d ) ROS levels were quantified by a fluorescence method using DCHFDA. ( e ) Transfection efficiency of SPT -siRNA was determined by quantifying the relative mRNA of SPT . Each measurement was made in duplicate, and the values are represented as mean ± SD from 3–4 cell preparations. NG and HG = 5 mM or 20 mM d -glucose; HG + PA = 20 mM d -glucose + palmitic acid; HG/ SPT -si and HG + PA/ SPT -si = SPT -siRNA transfected cells in 20 mM d -glucose or 20 mM d -glucose + palmitic acid; HG + PA/Myr = 20 mM d -glucose + palmitic acid + Myriocin; HG/SC = SPT -scrambled control RNA transfected cells in 20 mM d -glucose; L-Gl = 20 mM l -glucose. *p < 0.05 vs NG, # p < 0.05 vs HG and # **p < 0.05 vs HG + PA.
    Figure Legend Snippet: Inhibition of SPT and Rac1–Nox2–ROS signaling in HRECs. ( a ) SPT mRNA was quantified by qRT-PCR using β-actin as a house keeping gene. ( b ) Nox2 by using lucigenin as an electron acceptor and NADPH as a substrate and ( c ) Rac1 activity was determined by G-LISA colorimetric assay kit. ( d ) ROS levels were quantified by a fluorescence method using DCHFDA. ( e ) Transfection efficiency of SPT -siRNA was determined by quantifying the relative mRNA of SPT . Each measurement was made in duplicate, and the values are represented as mean ± SD from 3–4 cell preparations. NG and HG = 5 mM or 20 mM d -glucose; HG + PA = 20 mM d -glucose + palmitic acid; HG/ SPT -si and HG + PA/ SPT -si = SPT -siRNA transfected cells in 20 mM d -glucose or 20 mM d -glucose + palmitic acid; HG + PA/Myr = 20 mM d -glucose + palmitic acid + Myriocin; HG/SC = SPT -scrambled control RNA transfected cells in 20 mM d -glucose; L-Gl = 20 mM l -glucose. *p < 0.05 vs NG, # p < 0.05 vs HG and # **p < 0.05 vs HG + PA.

    Techniques Used: Inhibition, Quantitative RT-PCR, Activity Assay, Colorimetric Assay, Fluorescence, Transfection, Control

    Rac1–Nox2 colocalization and inhibition of SPT. ( a ) Rac1 and Nox2 were determined by immunofluorescence using DyLight green (green)-conjugated and Texas Red (red)-conjugated secondary antibodies, respectively. The line marker represents 20µm. ‘Arithmetic mean intensity’ (AMI) of ( b ) Nox2 and ( c ) Rac1 was quantified by Zeiss software, from 5 to 8 images/group. ( d ) Zeiss software module was used to calculate Pearson’s correlation coefficient between Rac1 and Nox2. The values in the graph are mean ± SD, obtained from 3–4 different cell preparations with multiple cells analyzed in each preparation. *p < 0.05 vs NG, # p < 0.05 vs HG and # **p < 0.05 vs HG + PA.
    Figure Legend Snippet: Rac1–Nox2 colocalization and inhibition of SPT. ( a ) Rac1 and Nox2 were determined by immunofluorescence using DyLight green (green)-conjugated and Texas Red (red)-conjugated secondary antibodies, respectively. The line marker represents 20µm. ‘Arithmetic mean intensity’ (AMI) of ( b ) Nox2 and ( c ) Rac1 was quantified by Zeiss software, from 5 to 8 images/group. ( d ) Zeiss software module was used to calculate Pearson’s correlation coefficient between Rac1 and Nox2. The values in the graph are mean ± SD, obtained from 3–4 different cell preparations with multiple cells analyzed in each preparation. *p < 0.05 vs NG, # p < 0.05 vs HG and # **p < 0.05 vs HG + PA.

    Techniques Used: Inhibition, Immunofluorescence, Marker, Software

    Rac1–Nox2–ROS signaling and SPT inhibition in retinal microvessels from diabetic mice. Retinal microvessels were analyzed for ( a ) Rac1 activity, ( b ) Nox2 activity, and ( c ) ROS by DCHFDA method. Each measurement was made in duplicate, and the values are means ± SD of 5–7 mice/group. Norm = normal mice on regular chow; Diab = diabetic mice on regular chow; HF-SD = diabetic mice on high fat chow; Diab /SPT -si or HF-SD/ SPT -si = SPT -siRNA administered diabetic mice on regular chow or high fat chow, respectively. *p < 0.05 vs Norm, # p < 0.05 vs Diab and # **p < 0.05 vs HF-SD.
    Figure Legend Snippet: Rac1–Nox2–ROS signaling and SPT inhibition in retinal microvessels from diabetic mice. Retinal microvessels were analyzed for ( a ) Rac1 activity, ( b ) Nox2 activity, and ( c ) ROS by DCHFDA method. Each measurement was made in duplicate, and the values are means ± SD of 5–7 mice/group. Norm = normal mice on regular chow; Diab = diabetic mice on regular chow; HF-SD = diabetic mice on high fat chow; Diab /SPT -si or HF-SD/ SPT -si = SPT -siRNA administered diabetic mice on regular chow or high fat chow, respectively. *p < 0.05 vs Norm, # p < 0.05 vs Diab and # **p < 0.05 vs HF-SD.

    Techniques Used: Inhibition, Activity Assay



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    arithmetic mean intensity (ami) of rac1 and nox2  (Carl Zeiss)
    90
    Carl Zeiss arithmetic mean intensity (ami) of rac1 and nox2
    Inhibition of SPT and <t>Rac1–Nox2–ROS</t> signaling in HRECs. ( a ) SPT mRNA was quantified by qRT-PCR using β-actin as a house keeping gene. ( b ) Nox2 by using lucigenin as an electron acceptor and NADPH as a substrate and ( c ) Rac1 activity was determined by G-LISA colorimetric assay kit. ( d ) ROS levels were quantified by a fluorescence method using DCHFDA. ( e ) Transfection efficiency of SPT -siRNA was determined by quantifying the relative mRNA of SPT . Each measurement was made in duplicate, and the values are represented as mean ± SD from 3–4 cell preparations. NG and HG = 5 mM or 20 mM d -glucose; HG + PA = 20 mM d -glucose + palmitic acid; HG/ SPT -si and HG + PA/ SPT -si = SPT -siRNA transfected cells in 20 mM d -glucose or 20 mM d -glucose + palmitic acid; HG + PA/Myr = 20 mM d -glucose + palmitic acid + Myriocin; HG/SC = SPT -scrambled control RNA transfected cells in 20 mM d -glucose; L-Gl = 20 mM l -glucose. *p < 0.05 vs NG, # p < 0.05 vs HG and # **p < 0.05 vs HG + PA.
    Arithmetic Mean Intensity (Ami) Of Rac1 And Nox2, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/arithmetic+mean+intensity+(ami)+of+rac1+and+nox2/arithmetic+intensity+mean/pmc09537524-191-8-18
    Average 90 stars, based on 1 article reviews
    arithmetic mean intensity (ami) of rac1 and nox2 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Inhibition of SPT and Rac1–Nox2–ROS signaling in HRECs. ( a ) SPT mRNA was quantified by qRT-PCR using β-actin as a house keeping gene. ( b ) Nox2 by using lucigenin as an electron acceptor and NADPH as a substrate and ( c ) Rac1 activity was determined by G-LISA colorimetric assay kit. ( d ) ROS levels were quantified by a fluorescence method using DCHFDA. ( e ) Transfection efficiency of SPT -siRNA was determined by quantifying the relative mRNA of SPT . Each measurement was made in duplicate, and the values are represented as mean ± SD from 3–4 cell preparations. NG and HG = 5 mM or 20 mM d -glucose; HG + PA = 20 mM d -glucose + palmitic acid; HG/ SPT -si and HG + PA/ SPT -si = SPT -siRNA transfected cells in 20 mM d -glucose or 20 mM d -glucose + palmitic acid; HG + PA/Myr = 20 mM d -glucose + palmitic acid + Myriocin; HG/SC = SPT -scrambled control RNA transfected cells in 20 mM d -glucose; L-Gl = 20 mM l -glucose. *p < 0.05 vs NG, # p < 0.05 vs HG and # **p < 0.05 vs HG + PA.

    Journal: Scientific Reports

    Article Title: Regulation of serine palmitoyl-transferase and Rac1–Nox2 signaling in diabetic retinopathy

    doi: 10.1038/s41598-022-20243-2

    Figure Lengend Snippet: Inhibition of SPT and Rac1–Nox2–ROS signaling in HRECs. ( a ) SPT mRNA was quantified by qRT-PCR using β-actin as a house keeping gene. ( b ) Nox2 by using lucigenin as an electron acceptor and NADPH as a substrate and ( c ) Rac1 activity was determined by G-LISA colorimetric assay kit. ( d ) ROS levels were quantified by a fluorescence method using DCHFDA. ( e ) Transfection efficiency of SPT -siRNA was determined by quantifying the relative mRNA of SPT . Each measurement was made in duplicate, and the values are represented as mean ± SD from 3–4 cell preparations. NG and HG = 5 mM or 20 mM d -glucose; HG + PA = 20 mM d -glucose + palmitic acid; HG/ SPT -si and HG + PA/ SPT -si = SPT -siRNA transfected cells in 20 mM d -glucose or 20 mM d -glucose + palmitic acid; HG + PA/Myr = 20 mM d -glucose + palmitic acid + Myriocin; HG/SC = SPT -scrambled control RNA transfected cells in 20 mM d -glucose; L-Gl = 20 mM l -glucose. *p < 0.05 vs NG, # p < 0.05 vs HG and # **p < 0.05 vs HG + PA.

    Article Snippet: The ‘Arithmetic mean intensity’ (AMI) of Rac1 and Nox2, and Pearson correlation coefficient between them were quantified using Zeiss software module .

    Techniques: Inhibition, Quantitative RT-PCR, Activity Assay, Colorimetric Assay, Fluorescence, Transfection, Control

    Rac1–Nox2 colocalization and inhibition of SPT. ( a ) Rac1 and Nox2 were determined by immunofluorescence using DyLight green (green)-conjugated and Texas Red (red)-conjugated secondary antibodies, respectively. The line marker represents 20µm. ‘Arithmetic mean intensity’ (AMI) of ( b ) Nox2 and ( c ) Rac1 was quantified by Zeiss software, from 5 to 8 images/group. ( d ) Zeiss software module was used to calculate Pearson’s correlation coefficient between Rac1 and Nox2. The values in the graph are mean ± SD, obtained from 3–4 different cell preparations with multiple cells analyzed in each preparation. *p < 0.05 vs NG, # p < 0.05 vs HG and # **p < 0.05 vs HG + PA.

    Journal: Scientific Reports

    Article Title: Regulation of serine palmitoyl-transferase and Rac1–Nox2 signaling in diabetic retinopathy

    doi: 10.1038/s41598-022-20243-2

    Figure Lengend Snippet: Rac1–Nox2 colocalization and inhibition of SPT. ( a ) Rac1 and Nox2 were determined by immunofluorescence using DyLight green (green)-conjugated and Texas Red (red)-conjugated secondary antibodies, respectively. The line marker represents 20µm. ‘Arithmetic mean intensity’ (AMI) of ( b ) Nox2 and ( c ) Rac1 was quantified by Zeiss software, from 5 to 8 images/group. ( d ) Zeiss software module was used to calculate Pearson’s correlation coefficient between Rac1 and Nox2. The values in the graph are mean ± SD, obtained from 3–4 different cell preparations with multiple cells analyzed in each preparation. *p < 0.05 vs NG, # p < 0.05 vs HG and # **p < 0.05 vs HG + PA.

    Article Snippet: The ‘Arithmetic mean intensity’ (AMI) of Rac1 and Nox2, and Pearson correlation coefficient between them were quantified using Zeiss software module .

    Techniques: Inhibition, Immunofluorescence, Marker, Software

    Rac1–Nox2–ROS signaling and SPT inhibition in retinal microvessels from diabetic mice. Retinal microvessels were analyzed for ( a ) Rac1 activity, ( b ) Nox2 activity, and ( c ) ROS by DCHFDA method. Each measurement was made in duplicate, and the values are means ± SD of 5–7 mice/group. Norm = normal mice on regular chow; Diab = diabetic mice on regular chow; HF-SD = diabetic mice on high fat chow; Diab /SPT -si or HF-SD/ SPT -si = SPT -siRNA administered diabetic mice on regular chow or high fat chow, respectively. *p < 0.05 vs Norm, # p < 0.05 vs Diab and # **p < 0.05 vs HF-SD.

    Journal: Scientific Reports

    Article Title: Regulation of serine palmitoyl-transferase and Rac1–Nox2 signaling in diabetic retinopathy

    doi: 10.1038/s41598-022-20243-2

    Figure Lengend Snippet: Rac1–Nox2–ROS signaling and SPT inhibition in retinal microvessels from diabetic mice. Retinal microvessels were analyzed for ( a ) Rac1 activity, ( b ) Nox2 activity, and ( c ) ROS by DCHFDA method. Each measurement was made in duplicate, and the values are means ± SD of 5–7 mice/group. Norm = normal mice on regular chow; Diab = diabetic mice on regular chow; HF-SD = diabetic mice on high fat chow; Diab /SPT -si or HF-SD/ SPT -si = SPT -siRNA administered diabetic mice on regular chow or high fat chow, respectively. *p < 0.05 vs Norm, # p < 0.05 vs Diab and # **p < 0.05 vs HF-SD.

    Article Snippet: The ‘Arithmetic mean intensity’ (AMI) of Rac1 and Nox2, and Pearson correlation coefficient between them were quantified using Zeiss software module .

    Techniques: Inhibition, Activity Assay